Impaired plasma lipid profiles in acute hepatitis

Abstract

The present study examined plasma lipid profiles in thirty patients suffered from acute viral hepatitis. Patients' blood samples were collected at both the debut and recovery of diseases. Thirty sex and age matched normal subjects were included as controls. Plasma total triglycerides (TG), total cholesterol, high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), apolipoprotein AI (ApoAI), apolipoprotein B (ApoB), lipoprotein (a) (Lp(a)), blood coagulation status including prothrombin complex activity and activated partial tromboplastin time (APTT), and hepatic functions were determined by the automatic biochemical analytical instrument. It demonstrated that plasma levels of total cholesterol, HDL-C and apoAI were significantly lower in the patients at the acute phase of hepatitis than those in normal subjects, whereas plasma levels of TG and LDL-C were obviously higher in the patients than in normal subjects (P < 0.05). Moreover, we demonstrated that patients' plasma levels of total cholesterol, LDL-C, HDL-C and apoAI were lower at the active phase of the diseases than at the recovering phase, which indicating that acute liver damage could significant influence lipid metabolism in vivo. No pathological changes of blood coagulation status occurred in these patients during the study as all selected patients had moderate hepatitis. It may conclude that examinations of plasma lipid profile could be considered as a clinical index to reflect liver damage in the active phase of hepatitis.

Introduction

Liver is the most important organ for the metabolism of lipids, lipoproteins and apolipoproteins. Under normal circumstances, most plasma endogenous lipids and lipoproteins are synthesized in the liver and then are secreted into the blood circulation [1, 2]. And plasma lipoproteins are also mainly catabolism by the liver to maintain the relative balance of lipid and lipoprotein metabolism in vivo [3]. It has been well documented that chronic liver dysfunction might interfere lipid metabolism in vivo and could change plasma lipid and lipoprotein patterns [4]. Acute hepatitis may be referred to an inflammatory process of the liver lasting less than six months. In China, the most common etiology of acute hepatitis is viral infection, in which hepatitis A and hepatitis E are the most common causes. In clinical, the courses of acute hepatitis may vary widely from mild symptom that does not require treatment to the fulminant hepatic failure that needs emergency liver transplantation. Acute viral hepatitis is more likely to be asymptomatic in younger people. In addition, acute hepatitis may occur less commonly with infections such as Epstein-Barr virus, cytomegalovirus, adenovirus, herpes simplex and Coxsackie virus or with other noninfectious reasons. It has been demonstrated that in the acute and/or chronic liver diseases, hepatic function could be impaired and the circulating lipids and lipoproteins are not only present in abnormal amounts but they frequently also have abnormal composition including electrophoretic mobility and appearance [4]. Previous studies pay more emphasis on changes of lipid metabolisms under chronic hepatitis and cirrhosis with or without hepatocellular carcinoma [5–7]. In the present study we followed plasma lipid and lipoprotein patterns of patients suffered from acute hepatitis to further explore the changes of lipid and lipoprotein profiles of the patients.

Materials and methods

Patients and normal subjects

Thirty cases of acute hepatitis patients who were admitted to the hospital's inpatients were subjected in the present study, 21 males and 9 females, aged from 19 to 81 years old. All patients had moderate hepatitis. Clinical diagnosis was applied according to the diagnostic criteria of "Viral Hepatitis Prevention Program" of the Chinese Society of Hepatology and Society of Infectious Diseases [8]. The characterization of the patients is listed in the table 1. Data concerning virus antigen and antibody, and viral loads in blood is shown in the Table 2. And there was no any patient with HIV positive in the present study. There was no patient received anti-viral drug therapy, blood transfusions or plasmapheresis. Duration of hospitalization all patients were given liver protective therapy and advised to have proper rest and go on rational diet. Some patients were given traditional Chinese medicine to improve liver function. In the present study there was no patient underwent liver biopsy. And up to now there was no patient was found to develop chronic active hepatitis. Thirty healthy subjects, 16 males and 14 females, aged from 20 to 63 years old, were included as controls. All normal subjects were confirmed by the negative results of serum biochemical tests, virus tests and B-type ultrasonic inspection to exclude hepatitis or other chronic liver diseases and metabolic diseases. Fasting blood samples were obtained and preserved at -70°C before further examination. The present study was approved by the local Ethics Committee.

Table 1 Characterization of patients and controls

Table 2 Viral tests of the patients

Laboratory analyses

Blood was collected from all subjects under standardised conditions after overnight fasting. Triglycerides (TG) and total cholesterol were determined by the endpoint detection (GPO-PAP Method, CHO-PAP Method). ApoA, apoB as well as Lp(a) were determined by the immunological turbidimetric method. All laboratory parameters were directly determined on an automatic biochemical analyzer (Olympus AU2700, Japan). Patients' coagulation status including prothrombin complex activity and activated partial tromboplastin time (APTT), were determined as a routine test and patients' coagulation status was at normal ranges.

Statistical analysis

Statistical analysis was performed with the GraphPad Prism version 5. Data are expressed as means ± SD. Differences between patients and healthy subjects were compared by the non-paired t-test. Lipid profiles in patients under different diseases phases were analyzed by the one-way ANOVA followed by the paired t-test. A p value less than 0.05 (P < 0.05) was considered as significant.

Results

As shown in figure 1, serum levels of total cholesterol, HDL-C and apoAI were significantly lower in the acute hepatitis patients compared to the normal subjects, whereas serum levels of TG and LDL-C were obviously higher in the patients than in normal subjects (P < 0.05). Moreover, we demonstrated that patients' serum levels of total cholesterol, LDL-cholesterol, HDL-cholesterol and apoAI were lower at the active phase of the diseases than at the recovering phase (Figure 2). There is no statistical significant correlation between the plasma viraemia and the changes of lipid profiles (data not shown).

Figure 1

Plasma lipid levels in acute hepatitis patients and in normal subjects. Data are mean ± SD. * P < 0.05 and *** P < 0.001 vs. normal subjects.

Figure 2

Lipid profiles in patients at different phases of the diseases. Day 0 represents the debut of disease, day 10 and day 20 represent the treatment days. Data are mean ± SD. * P < 0.05 and ** P < 0.01.

Discussion

Liver is one of the most important organs for the metabolism of plasma apolipoproteins, endogenous lipids and lipoproteins. Most plasma apolipoproteins, endogenous lipids and lipoproteins are synthesized by the liver [1, 2], which depends on the integrity of cellular functions of liver [2, 3]. As in blood circulation lipids do not dissolve in plasma, they need in combination with different apolipoproteins to form lipoproteins that may transfer endogenous or exogenous lipids to different organs or tissues for further metabolism. Under normal physiological conditions, liver plays an important role to regulate lipid and lipoprotein metabolisms. Liver not only synthesizes and secretes endogenous lipoprotein, synthesis of key enzyme for the LDL metabolism, i.e., lecithin cholesterol acyltransferase (LCAT), hepatic lipase and apolipoproteins, but also regulates catabolism of various plasma lipoproteins via hepatic cellular surface lipoprotein receptors, which may maintain relative equilibrium of plasma lipids and lipoproteins in vivo [9, 10]. These processes could be interfered or impaired when hepatic cellular damage, which leads to an alteration of plasma lipid and lipoprotein patterns. And syntheses of cholesterol, triglycerides, apoAI, apoB and Lp(a) could be changed and their plasma concentrations will be altered correspondingly [11]. Therefore, examination of plasma lipid and lipoprotein levels will be helpful to evaluate the extent of the hepatic damage. It is well known that plasma levels of cytokines, lipid peroxides and anti-oxidant status could be changed under acute or chronic hepatitis [12–14], which may also interfere the lipid metabolism in vivo.

In the present results we demonstrated that plasma levels of total cholesterol, HDL-C and apoAI were significantly lower in the patients at the acute phase of hepatitis than those in normal subjects, whereas plasma levels of triglycerides and LDL-C were obviously higher in the patients than in normal subjects. And patients' plasma levels of total cholesterol, LDL-C, HDL-C and apoAI were lower at the active phase of the diseases than at the recovering phase, which indicating that acute liver damage could significant influence lipid metabolism in vivo. It is well known that severe acute hepatitis is an serious acute inflammatory process that leads to a hepatic cellular necrosis [15]. It has been reported when acute hepatitis occurred, liver function could be severely damaged, which could significantly inhibit hepatic apoAI synthesis [16]. Serum levels of apoAI, apoAII and total cholesterol were negatively correlated to serious of liver damage [17–19]. According to our results and together with the literatures [20, 21] apoAI and HDL-C could be considered as the best indicator to evaluate accurately the liver damage. The combination of plasma levels of apoAI and HDL-C together with prothrombin activity and serum levels of bilirubin and transaminase could be a best index for evaluating the prognosis of acute hepatitis.