Research

Lactic acid bacteria affect serum cholesterol levels, harmful fecal enzyme activity, and fecal water content

Do Kyung Lee¹ , Seok Jang¹ , Eun Hye Baek¹ , Mi Jin Kim¹ , Kyung Soon Lee² , Hea Soon Shin³ , Myung Jun Chung⁴ , Jin Eung Kim⁴ , Kang Oh Lee⁵ and Nam Joo Ha¹

¹  Department of Pharmacy, Sahmyook University, Seoul 139-742, Republic of Korea

²  Department of Nursing, Sahmyook University, Seoul 139-742, Republic of Korea

³  College of Pharmacy, Duksung Women's University, Seoul 132-714, Republic of Korea

⁴  Cellbiotech Co Ltd, Seoul 157-030, Republic of Korea

⁵  Department of Life Science, Sahmyook University, Seoul 139-742, Republic of Korea

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Lipids in Health and Disease 2009, 8:21doi:10.1186/1476-511X-8-21

Published:	11 June 2009

Abstract

Background

Lactic acid bacteria (LAB) are beneficial probiotic organisms that contribute to improved nutrition, microbial balance, and immuno-enhancement of the intestinal tract, as well as lower cholesterol. Although present in many foods, most trials have been in spreads or dairy products. Here we tested whether Bifidobacteria isolates could lower cholesterol, inhibit harmful enzyme activities, and control fecal water content.

Methods

In vitro culture experiments were performed to evaluate the ability of Bifidobacterium spp. isolated from healthy Koreans (20~30 years old) to reduce cholesterol-levels in MRS broth containing polyoxyethanylcholesterol sebacate. Animal experiments were performed to investigate the effects on lowering cholesterol, inhibiting harmful enzyme activities, and controlling fecal water content. For animal studies, 0.2 ml of the selected strain cultures (10⁸~10⁹ CFU/ml) were orally administered to SD rats (fed a high-cholesterol diet) every day for 2 weeks.

Results

B. longum SPM1207 reduced serum total cholesterol and LDL levels significantly (p < 0.05), and slightly increased serum HDL. B. longum SPM1207 also increased fecal LAB levels and fecal water content, and reduced body weight and harmful intestinal enzyme activities.

Conclusion

Daily consumption of B. longum SPM1207 can help in managing mild to moderate hypercholesterolemia, with potential to improve human health by helping to prevent colon cancer and constipation.