Figure 1.

Effects of the CCl₄ and flavonoids on the hepatocyte death. The cells (10⁶/ml) were incubated at 37°C in Eagle medium containing 10–100 mM CCl₄ for 2 h. Control cells were incubated in the presence of 30 mM ethanol for 2 h. Hepatocytes were incubated in the presence of the 250–750 μg/ml chamiloflan, or 10–60 μM AP7Glu for 4 h. The time of the control cell suspension incubation (in the presence of 30 mM ethanol) was 4 h. To determine the cells viability, the trypan blue exclusion into hepatocytes and LDH release into supernatant have been studied using commercially available kits. Results are mean ± S.E. of six experiments performed in duplicate. * P < 0.05, CCl₄ -treated vs. control.