Table 4

Fatty Acid Composition of Rat Red Gastrocnemius After Dietary Supplementation with 22:6n-3 and 22:5n-6*
		Artificially reared

	Dam reared (n = 8)	AR-LA (n = 7)	AR-DPA (n = 7)	AR-DHA+DPA (n = 8)	AR-DHA (n = 7)

Fatty acids	(weight % of total fatty acids)
12:0	2.10 ± 0.84^a	0.96 ± 0.53^b	1.07 ± 0.76^ab	1.00 ± 0.88^b	0.84 ± 0.41^b
14:0	2.44 ± 0.54^a	1.62 ± 0.55^ab	1.75 ± 0.69^ab	1.60 ± 0.69^ab	1.48 ± 0.48^b
16:0 DMA	1.86 ± 0.44	1.37 ± 0.41	1.41 ± 0.46	1.71 ± 0.64	1.89 ± 0.62
16:0	22.1 ± 1.1^a	22.5 ± 1.1^ab	23.8 ± 1.5^b	22.7 ± 0.8^ab	22.2 ± 1.1^ab
18:0 DMA	0.85 ± 0.26^a	0.44 ± 0.19^b	0.49 ± 0.16^ab	0.57 ± 0.29^ab	0.69 ± 0.29^ab
18:0	11.9 ± 0.8	10.7 ± 2.1	9.9 ± 2.5	10.6 ± 2.3	10.5 ± 1.4
20:0	0.08 ± 0.02^a	0.05 ± 0.01^b	0.05 ± 0.02^b	0.06 ± 0.01^b	0.06 ± 0.01^ab
22:0	0.08 ± 0.03	0.06 ± 0.01	0.05 ± 0.02	0.06 ± 0.02	0.08 ± 0.03
24:0	0.17 ± 0.02^a	0.10 ± 0.02^bc	0.09 ± 0.03^b	0.13 ± 0.02^cd	0.16 ± 0.03^ad
Total saturates	41.6 ± 1.0^a	37.8 ± 1.7^b	38.6 ± 1.0^b	38.4 ± 1.6^b	37.9 ± 1.6^b

16:1 n-7	3.44 ± 0.63	3.7 ± 1.8	4.1 ± 2.0	3.2 ± 1.2	3.19 ± 0.86
18:1 DMA	0.20 ± 0.06^a	0.23 ± 0.09^ab	0.24 ± 0.12^ab	0.33 ± 0.14^ab	0.39 ± 0.14^b
18:1 n-9	10.6 ± 1.7^a	18.6 ± 5.7^ab	19.6 ± 7.1^b	17.6 ± 7.0^ab	17.7 ± 4.9^ab
18:1 n-7	3.34 ± 0.19	3.70 ± 0.55	3.59 ± 0.30	3.70 ± 0.39	3.84 ± 0.40
20:1 n-9	0.07 ± 0.02^a	0.13 ± 0.03^b	0.14 ± 0.04^b	0.12 ± 0.05^b	0.11 ± 0.03^ab
24:1 n-9	0.16 ± 0.04	0.15 ± 0.09	0.13 ± 0.05	0.18 ± 0.07	0.17 ± 0.03
Total monounsaturates	17.9 ± 2.3	26.5 ± 7.7	27.8 ± 9.1	25.2 ± 8.3	25.4 ± 5.6

18:2 n-6	14.5 ± 1.5^a	12.1 ± 1.7^b	10.3 ± 1.4^b	10.2 ± 0.9^b	10.3 ± 1.0^b
18:3 n-6	0.03 ± 0.01^ab	0.04 ± 0.01^a	0.03 ± 0.01^ab	0.03 ± 0.01^b	0.03 ± 0.01^ab
20:2 n-6	0.08 ± 0.05^a	0.04 ± 0.01^b	0.04 ± 0.01^b	0.04 ± 0.01^b	0.04 ± 0.01^ab
20:3 n-6	0.43 ± 0.05	0.46 ± 0.12	0.43 ± 0.11	0.46 ± 0.15	0.42 ± 0.08
20:4 n-6	8.9 ± 1.0	11.8 ± 3.1	9.8 ± 3.8	9.2 ± 2.7	9.1 ± 1.5
22:4 n-6	0.38 ± 0.04^a	0.92 ± 0.24^b	0.62 ± 0.23^c	0.39 ± 0.08^a	0.37 ± 0.07^a
22:5 n-6	0.53 ± 0.07^a	5.9 ± 1.9^b	8.1 ± 2.8^b	1.97 ± 0.57^a	1.30 ± 0.43^a
Total n-6 PUFA	24.8 ± 2.2^abc	31.3 ± 6.3^b	29.3 ± 8.0^bc	22.3 ± 3.8^ac	21.6 ± 2.5^a

18:3 n-3	0.35 ± 0.07^a	0.02 ± 0.01^b	0.02 ± 0.01^b	0.03 ± 0.01^b	0.02 ± 0.01^b
20:5 n-3	0.13 ± 0.03^a	-	-	0.03 ± 0.01^b	0.04 ± 0.02^b
22:5 n-3	1.18 ± 0.11^a	0.11 ± 0.03^b	0.09 ± 0.03^b	0.17 ± 0.05^b	0.19 ± 0.06^b
22:6 n-3	11.6 ± 1.0^a	0.89 ± 0.25^b	1.14 ± 0.50^b	11.0 ± 3.7^a	12.2 ± 2.3^a
Total n-3 PUFA	13.2 ± 1.1^a	1.02 ± 0.27^b	1.24 ± 0.51^b	11.2 ± 3.7^a	12.4 ± 2.4^a

Total PUFA	38.1 ± 2.7	32.3 ± 6.5	30.5 ± 8.2	33.5 ± 7.3	34.0 ± 4.7
Total HUFA	23.1 ± 2.0	20.1 ± 5.4	20.2 ± 7.2	23.2 ± 4.2	23.6 ± 4.2
% of n-3 HUFA in HUFA	55.8 ± 1.8^a	5.02 ± 0.30^b	6.5 ± 2.8^b	47.7 ± 3.3^c	52.4 ± 3.0^a
Total FA (mg/g)	13.5 ± 1.7	16.4 ± 6.5	16.7 ± 6.7	17.2 ± 8.1	14.3 ± 2.9

*Values are mean ± SD. Values in a row not sharing a roman superscript are significantly different at P < 0.05 by Tukey's honestly significantly difference test after a significant F-value (P < 0.05) by one-way ANOVA. AR-LA, 18:2n-6 artificial reared diet; AR-DPAn-6, 22:5n-6 artificial reared diet; AR-DHA+DPAn-6, 22:6n-3 + 22:5n-6 artificial reared diet; AR-DHA, 22:6n-3 artificial reared diet; DMA, dimethyl acetal; PUFA, polyunsaturated fatty acids; HUFA, highly unsaturated fatty acids (≥ 20 carbons, ≥ 3 carbon-carbon double bonds).
Stark et al. Lipids in Health and Disease 2007 6:13 doi:10.1186/1476-511X-6-13