Table 3

Fatty Acid Composition of Rat Soleus After Dietary Supplementation with 22:6n-3 and 22:5n-6*
		Artificially reared

	Dam reared (n = 7)	AR-LA (n = 7)	AR-DPAn-6 (n = 7)	AR-DHA+DPAn-6 (n = 8)	AR-DHA (n = 7)

Fatty acids	(weight % of total fatty acids)
12:0	6.3 ± 2.2^a	2.55 ± 0.46^b	2.53 ± 0.45^b	2.55 ± 0.70^b	2.63 ± 0.73^b
14:0	5.1 ± 1.1^a	3.02 ± 0.27^b	3.03 ± 0.25^b	3.12 ± 0.40^b	3.07 ± 0.51^b
16:0 DMA	0.93 ± 0.42	0.75 ± 0.25	0.77 ± 0.37	0.66 ± 0.25	0.67 ± 0.36
16:0	20.1 ± 2.0	20.6 ± 1.6	20.8 ± 0.9	21.6 ± 1.0	20.3 ± 0.6
18:0 DMA	0.54 ± 0.27	0.26 ± 0.12	0.30 ± 0.17	0.28 ± 0.15	0.27 ± 0.15
18:0	8.4 ± 2.2	6.7 ± 1.7	6.6 ± 1.7	5.6 ± 1.4	6.4 ± 2.6
20:0	0.10 ± 0.02^a	0.05 ± 0.02^b	0.05 ± 0.01^b	0.05 ± 0.02^b	0.06 ± 0.03^b
22:0	0.11 ± 0.08	0.04 ± 0.02	0.05 ± 0.02	0.05 ± 0.05	0.05 ± 0.03
24:0	0.15 ± 0.09^a	0.06 ± 0.02^b	0.07 ± 0.03^ab	0.08 ± 0.06^ab	0.09 ± 0.05^ab
Total saturates	41.6 ± 0.8^a	34.0 ± 2.1^b	34.2 ± 2.4^b	34.0 ± 1.6^b	33.5 ± 2.1^b

16:1 n-7	7.3 ± 1.6	7.8 ± 1.4	7.6 ± 1.0	8.3 ± 1.8	7.5 ± 2.3
18:1 DMA	0.08 ± 0.06	0.07 ± 0.02	0.08 ± 0.04	0.10 ± 0.11	0.08 ± 0.05
18:1 n-9	17.3 ± 2.5^a	31.2 ± 3.7^b	31.6 ± 4.2^b	32.8 ± 3.8^b	32.1 ± 5.5^b
18:1 n-7	3.93 ± 0.36	4.55 ± 0.61	4.40 ± 0.50	4.27 ± 0.45	4.38 ± 0.33
20:1 n-9	0.09 ± 0.02^a	0.19 ± 0.03^b	0.18 ± 0.05^b	0.20 ± 0.04^b	0.20 ± 0.05^b
24:1 n-9	0.13 ± 0.04	0.12 ± 0.04	0.11 ± 0.04	0.12 ± 0.05	0.14 ± 0.07
Total monounsaturates	28.8 ± 4.2^a	44.0 ± 5.2^b	44.0 ± 5.1^b	45.8 ± 5.3^b	44.4 ± 7.9^b

18:2 n-6	13.1 ± 1.9^a	8.4 ± 1.0^b	8.24 ± 0.77^b	7.82 ± 0.52^b	8.4 ± 1.2^b
18:3 n-6	0.03 ± 0.01^a	0.03 ± 0.01^b	0.024 ± 0.004^b	0.022 ± 0.004^ab	0.022 ± 0.004^ab
20:2 n-6	0.06 ± 0.01^a	0.04 ± 0.01^b	0.04 ± 0.01^b	0.04 ± 0.01^b	0.05 ± 0.01^ab
20:3 n-6	0.27 ± 0.06	0.22 ± 0.06	0.23 ± 0.07	0.20 ± 0.05	0.20 ± 0.07
20:4 n-6	5.8 ± 1.7	6.2 ± 1.8	5.4 ± 1.7	4.2 ± 1.4	4.9 ± 2.4
22:4 n-6	0.40 ± 0.09^ab	0.56 ± 0.14^a	0.41 ± 0.11^ab	0.27 ± 0.03^b	0.31 ± 0.14^b
22:5 n-6	0.27 ± 0.10^a	2.26 ± 0.73^b	3.03 ± 0.99^b	0.78 ± 0.29^a	0.56 ± 0.32^a
Total n-6 PUFA	19.9 ± 3.5^a	17.7 ± 3.5^ab	17.3 ± 3.2^ab	13.3 ± 1.9^b	14.4 ± 3.7^b

18:3 n-3	0.78 ± 0.23^a	0.03 ± 0.01^b	0.023 ± 0.003^b	0.03 ± 0.01^b	0.04 ± 0.04^b
20:5 n-3	0.08 ± 0.02^a	-	-	0.02 ± 0.01^b	0.025 ± 0.003^b
22:5 n-3	0.59 ± 0.19^a	0.04 ± 0.02^b	0.04 ± 0.01^b	0.06 ± 0.03^b	0.08 ± 0.03^b
22:6 n-3	4.3 ± 1.7^a	0.32 ± 0.14^b	0.44 ± 0.32^b	3.2 ± 2.0^a	3.7 ± 2.1^a
Total n-3 PUFA	5.7 ± 1.7^a	0.40 ± 0.16^b	0.50 ± 0.32^b	3.3 ± 2.0^c	3.8 ± 2.1^ac

Total PUFA	25.6 ± 4.5^a	18.1 ± 3.7^b	17.8 ± 3.4^b	16.6 ± 3.9^b	18.3 ± 5.7^b
Total HUFA	11.7 ± 3.6	9.6 ± 2.8	9.5 ± 3.0	8.7 ± 3.6	9.8 ± 5.0
% of n-3 HUFA in HUFA	41.3 ± 6.3^a	3.8 ± 0.6^b	4.9 ± 2.7^b	35.0 ± 6.9^a	38.2 ± 3.12^a
Total FA (mg/g)	26.1 ± 8.5	34.5 ± 10.6	37.4 ± 15.6	43.2 ± 17.2	40.0 ± 19.5

*Values are mean ± SD. Values in a row not sharing a roman superscript are significantly different at P < 0.05 by Tukey's honestly significantly difference test after a significant F-value (P < 0.05) by one-way ANOVA. AR-LA, 18:2n-6 artificial reared diet; AR-DPAn-6, 22:5n-6 artificial reared diet; AR-DHA+DPAn-6, 22:6n-3 + 22:5n-6 artificial reared diet; AR-DHA, 22:6n-3 artificial reared diet; DMA, dimethyl acetal; PUFA, polyunsaturated fatty acids; HUFA, highly unsaturated fatty acids (≥ 20 carbons, ≥ 3 carbon-carbon double bonds).
Stark et al. Lipids in Health and Disease 2007 6:13 doi:10.1186/1476-511X-6-13