Reduction in intracellular lipid accumulation in adipocytes by heparan sulfate inhibitors is concentration-dependent. 4-MUmb (solid bars) or pNP-Xyl (hatched bars) was added to 3T3-L1 cells at the indicated concentrations concurrently with differentiation reagents as in Fig. 5. Cells were then fixed with 10% formaldehyde, stained with 0.1% Oil Red O, and cell-associated lipophilic dye was extracted with 0.1 N HCl and quantitated by spectrophotometry. Data shown represents an average of three experiments.
Wilsie et al. Lipids in Health and Disease 2005 4:2 doi:10.1186/1476-511X-4-2