Reduction of cell surface heparan sulfate glycosaminoglycans significantly reduces intracellular lipid accumulation in adipocytes. 3 mM 4-MUmb or pNP-Xyl was added to 3T3-L1 cells concurrently with differentiation reagents as in Fig. 5. Cells were then fixed with 10% formaldehyde, stained with 0.1% Oil Red O and photographed with phase contrast optics (A; magnification, 400×). (B), after image capture, cell-associated Oil Red O was extracted with 0.1 N HCl and dye was quantitated by spectrophotometry. Asterisk indicates a statistically significant difference compared to untreated adipocytes (p < 0.01).
Wilsie et al. Lipids in Health and Disease 2005 4:2 doi:10.1186/1476-511X-4-2