Expression of VLDL-R and LRP increases following adipocyte differentiation. Pre-adipocyte 3T3-L1 cells were incubated for 4 d in the presence (+) or absence (-) of 100 ng/ml dexamethasone, 100 μg/ml 3-isobutyl-1-methylxanthine, and 1 μg/ml insulin, followed by 1 μg/ml insulin for an additional 4–8 d. Total cellular protein was obtained by detergent extraction, equal amounts of protein (20 μg/lane) were separated by SDS-PAGE and immunoblotted with anti-VLDL-R polyclonal IgG (4 μg/ml), or anti-LRP (1:2000) or anti-RAP (1:2000) antisera. The chemiluminescence image was quantitated by densitometry. Values obtained for non-treated cells were assigned as 100% for comparison with treated cells. Data shown is representative of 3 separate experiments.
Wilsie et al. Lipids in Health and Disease 2005 4:2 doi:10.1186/1476-511X-4-2