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Open Access Research

Isolation of a novel amylase and lipase-producing Pseudomonas luteola strain: study of amylase production conditions

Lamia Khannous1, Mouna Jrad2, Mouna Dammak3, Ramzi Miladi3, Nour Chaaben3, Bassem Khemakhem2, Néji Gharsallah2 and Imen Fendri1*

Author Affiliations

1 Faculté des Sciences de Sfax, Unité de recherche Toxicologie- Microbiologie Environnementale et Santé, Université de, Sfax, Tunisia

2 Laboratoire de Biotechnologie des Plantes, Faculté des Sciences de Sfax, Université de Sfax, Sfax, Tunisia

3 Département de génie biologique, Ecole Nationale d’Ingénieurs de Sfax, Université de Sfax, Sfax, Tunisia

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Lipids in Health and Disease 2014, 13:9  doi:10.1186/1476-511X-13-9

Published: 9 January 2014

Abstract

An amylase and lipase producing bacterium (strain C2) was enriched and isolated from soil regularly contaminated with olive washing wastewater in Sfax, Tunisia. Cell was aerobic, mesophilic, Gram-negative, motile, non-sporulating bacterium, capable of growing optimally at pH 7 and 30°C and tolerated maximally 10% (W/V) NaCl. The predominant fatty acids were found to be C18:1ω7c (32.8%), C16:1ω7c (27.3%) and C16:0 (23.1%). Phylogenetic analysis of the 16S rRNA gene revealed that this strain belonging to the genus Pseudomonas. Strain C2 was found to be closely related to Pseudomonas luteola with more than 99% of similarity. Amylase optimization extraction was carried out using Box Behnken Design (BBD). Its maximal activity was found when the pH and temperature ranged from 5.5 to 6.5 and from 33 to 37°C, respectively. Under these conditions, amylase activity was found to be about 9.48 U/ml.

Keywords:
Pseudomonas luteola; Amylase; Lipase; Box Behnken Design