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Open Access Research

Association between polymorphisms in the flanking region of the TAFI gene and atherosclerotic cerebral infarction in a Chinese population

You Li1, Zhiliang Zeng2, Jianghao Zhao2, Guoda Ma1, Lili Cui1, Hua Tao2, Zhijun Lin2, Yanyan Chen1, Bin Zhao12, Yusen Chen2* and Keshen Li1*

Author Affiliations

1 Guangdong Key Laboratory of Age-Related Cardiac and Cerebral Diseases, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China

2 Department of Neurology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China

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Lipids in Health and Disease 2014, 13:80  doi:10.1186/1476-511X-13-80

Published: 13 May 2014

Abstract

Background

Atherosclerosis is the leading etiologic factor of Atherosclerotic Cerebral Infarction (ACI). Previous studies have shown that thrombin activatable fibrinolysis inhibitor (TAFI) may play an important role in the occurrence of acute cerebral infarction, and the levels of TAFI are affected by several single nucleotide polymorphisms (SNPs) located in the regulatory and coding regions of the gene encoding TAFI. The present study aimed to determine whether polymorphisms (TAFI –2345 2G/1G, –1690 A/G, –438 A/G, +1583 A/T) of the TAFI gene were associated with ACI in a Han Chinese population.

Methods

The variant genotypes were identified by restriction fragment length polymorphism (RFLP) and allele-specific polymerase chain reactions (AS-PCR) in 225 patients with ACI and 184 age-matched healthy individuals.

Results

There was a significant difference in the genotype and allele frequencies of TAFI –2345 2G/1G and −1690 A/G polymorphisms between the ACI and control subjects. Further stratification analysis by gender revealed that the presence of the –438 AA genotype and the A allele conferred a higher risk of developing ACI in male patients (p < 0.05). Haplotype analysis demonstrated that four haplotypes of TAFI are significantly associated with ACI.

Conclusions

Our study provides preliminary evidence that the TAFI –2345 2G/1G and –1690 A/G polymorphisms are associated with ACI susceptibility in a Han Chinese population.

Keywords:
Thrombin activatable fibrinolysis inhibitor (TAFI); Atherosclerotic Cerebral Infarction (ACI); Polymorphism