Overexpression of stearoyl-CoA desaturase 1 in bone marrow mesenchymal stem cells enhance the expression of induced endothelial cells
1 Department of Transfusion, Shanghai First People’s Hospital, School of Medicine, Shanghai Jiao Tong University, 100, Haining Road, Shanghai 200080, China
2 Department of Orthopedics, Shanghai First People’s Hospital, School of Medicine, Shanghai Jiao Tong University, 100, Haining Road, Shanghai 200080, China
3 Department of Orthopedics, Shanghai First People’s Hospital Songjiang Branch, School of Medicine, Shanghai Jiao Tong University, 746, Zhongshan Middle Road, Shanghai 201600, China
Lipids in Health and Disease 2014, 13:53 doi:10.1186/1476-511X-13-53Published: 20 March 2014
Bone marrow mesenchymal stem cells (BM-MSCs) are capable of differentiating into endothelial cells in vitro and acquire major characteristics of mature endothelial-like expression of vWF and CD31. SFAs and lipid oxidation products have been linked with postprandial endothelial dysfunction. Consumption of SFAs impairs arterial endothelial function, while a Mediterranean-type MUFA-diet has a beneficial effect on endothelial function by producing a decrease in levels of vWF, TFPI and PAI-1. Stearoyl-CoA desaturase 1 (SCD1), which converts SFA to MUFA, is involved in the cellular biosynthesis of MUFAs from SFA substrates. High expression of SCD1 is corresponded with low rates of fatty acid oxidation, therefore it might reduce inflammatory responses and be beneficial for the growth of induced endothelial cells. Overexpression of SCD1 in BM-MSCs might increase the growth of induced endothelial cells. The goal of this research is to study the relationship between overexpression of SCD1 and the expression of induced endothelial cells in BM-MSCs in vitro.
The gene SCD1 was integrated into a lentiviral vector, and then 293 T cells were transfected by the connected product to produce a packaged virus. BM-MSCs were infected by the packaged virus. Cell culture and endothelial induction were performed. Fluorescent quantitative PCR of CD31, vWF and VE-cad was performed after 1 week and 2 weeks to test the growth of induced endothelial cells.
The mRNA amount of CD31, vWF and VE-cad of the SCD1 overexpressed group was statistically higher than that of the empty vector (EV) group and that of the normal group after 1 week and 2 weeks, respectively (p < 0.05). Immunocytochemical staining of CD31 or vWF was detected by visualizing red color.
This study suggested that overexpression of SCD1 in BM-MSCs could increase the expression of induced endothelial cells in vitro.