Open Access Research

Oligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pups

Laís Vales Mennitti1, Lila Missae Oyama3, Juliana Lopez de Oliveira3, Ana Claudia Losinskas Hachul3, Aline Boveto Santamarina3, Aline Alves de Santana3, Marcos Hiromu Okuda3, Eliane Beraldi Ribeiro3, Claudia Maria da Penha Oller do Nascimento3 and Luciana Pellegrini Pisani2*

Author Affiliations

1 Programa de Pós Graduação Interdisciplinar em Ciências da Saúde, Universidade Federal de São Paulo, Santos, SP, Brazil

2 Departamento de Biociências, Instituto de Saúde e Sociedade, Universidade Federal de São Paulo, Rua Silva Jardim, 136, Térreo, Vila Mathias, Santos, SP, Brazil

3 Departamento Fisiologia, Disciplina de Fisiologia da Nutrição, Escola Paulista de Medicina, Universidade Federal de São Paulo, Rua Botucatu, 862, 2° andar, Vila Clementino, São Paulo, SP, Brazil

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Lipids in Health and Disease 2014, 13:26  doi:10.1186/1476-511X-13-26

Published: 5 February 2014

Abstract

Background

Previously, we showed that the intake of trans fatty acids during pregnancy and lactation triggers a pro-inflammatory status in the offspring. On the other hand, prebiotics can alter the intestinal environment, reducing serum lipopolysaccharides (LPS) concentrations. This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.

Methods

On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF). Diets were maintained during pregnancy and lactation. At birth, 7th, 14th and 21th, pups were weighed and length was measured. Serum concentrations of LPS and free fatty acids (FFA) were performed by specific kits. Bacterial DNA present in faeces was determined by real-time PCR. Data were expressed as mean ± standard error of the mean and the statistical analysis was realized by ANOVA two-way and ANOVA for repeated measures. p < 0.05 was considered significant.

Results

We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp. on faeces of the CF group in relation to C group.

Conclusion

In conclusion, dam’s diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups.

Keywords:
Hydrogenated vegetable fat; Oligofructose; Corporal composition; Lipopolysaccharides; Lactobacillus spp; Pregnancy; Lactation