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Acerola (Malpighia emarginata DC.) juice intake protects against alterations to proteins involved in inflammatory and lipolysis pathways in the adipose tissue of obese mice fed a cafeteria diet

Fernando Milanez Dias2, Daniela Dimer Leffa1, Francine Daumann1, Schérolin de Oliveira Marques2, Thais F Luciano2, Jonathan Correa Possato2, Aline Alves de Santana3, Rodrigo Xavier Neves4, José Cesar Rosa5, Lila Missae Oyama3, Bruno Rodrigues6, Vanessa Moraes de Andrade1, Cláudio Teodoro de Souza2 and Fabio Santos de Lira7*

Author Affiliations

1 Laboratory of Molecular and Cellular Biology, Graduate Programme of Health Sciences, Department of Health Sciences, Universidade do Extremo Sul Catarinense, UNESC, 888.06-000 Criciúma, SC, Brazil

2 Laboratory of Exercise Biochemistry and Physiology, Graduate Programme of Health Sciences, Department of Health Sciences, Universidade do Extremo Sul Catarinense, UNESC, 888.06-000 Criciúma, SC, Brazil

3 Departamento de Fisiologia, Disciplina de Fisiologia da Nutrição, Universidade Federal de São Paulo (UNIFESP), 04023-060 São Paulo, SP, Brazil

4 Cancer Metabolism Research Group, Institute of Biomedical Sciences, University of Sao Paulo (USP), São Paulo, Brazil

5 Immunometabolism Research Group, Institute of Biomedical Sciences, University of São Paulo (USP), São Paulo, Brazil

6 Laboratório do Movimento Humano, Universidade São Judas Tadeu, São Paulo, SP, Brazil

7 Immunometabolism Research Group, Department of Physical Education, Universidade Estadual Paulista, UNESP, Rua Roberto Simonsen, 305, 19060-900 Presidente Prudente, SP, Brazil

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Lipids in Health and Disease 2014, 13:24  doi:10.1186/1476-511X-13-24

Published: 4 February 2014

Abstract

Background

Obesity has been studied as a metabolic and an inflammatory disease and is characterized by increases in the production of pro-inflammatory adipokines in the adipose tissue.

To elucidate the effects of natural dietary components on the inflammatory and metabolic consequences of obesity, we examined the effects of unripe, ripe and industrial acerola juice (Malpighia emarginata DC.) on the relevant inflammatory and lipolysis proteins in the adipose tissue of mice with cafeteria diet-induced obesity.

Materials/methods

Two groups of male Swiss mice were fed on a standard diet (STA) or a cafeteria diet (CAF) for 13 weeks. Afterwards, the CAF-fed animals were divided into five subgroups, each of which received a different supplement for one further month (water, unripe acerola juice, ripe acerola juice, industrial acerola juice, or vitamin C) by gavage. Enzyme-linked immunosorbent assays, Western blotting, a colorimetric method and histology were utilized to assess the observed data.

Results

The CAF water (control obese) group showed a significant increase in their adiposity indices and triacylglycerol levels, in addition to a reduced IL-10/TNF-α ratio in the adipose tissue, compared with the control lean group. In contrast, acerola juice and Vitamin C intake ameliorated the weight gain, reducing the TAG levels and increasing the IL-10/TNF-α ratio in adipose tissue. In addition, acerola juice intake led to reductions both in the level of phosphorylated JNK and to increases in the phosphorylation of IκBα and HSLser660 in adipose tissue.

Conclusions

Taken together, these results suggest that acerola juice reduces low-grade inflammation and ameliorates obesity-associated defects in the lipolytic processes.

Keywords:
Cafeteria diet; Malpighia emarginata DC; Adipose tissue; Inflammation; Lipolysis