Nonalcoholic steatohepatitis is associated with an atherogenic lipoprotein subfraction profile
1 Gastrointestinal Unit, Massachusetts General Hospital, Boston, MA, USA
2 Department of Pathology, Massachusetts General Hospital, Boston, MA, USA
3 Department of Internal Medicine, Bon Secours Richmond Health System, Richmond, VA, USA
4 MGH Biostatistics Center, Massachusetts General Hospital, Boston, MA, USA
5 Harvard Medical School, Boston, MA, USA
6 Children’s Hospital Oakland Research Institute, Oakland, USA
Lipids in Health and Disease 2014, 13:100 doi:10.1186/1476-511X-13-100Published: 21 June 2014
Nonalcoholic steatohepatitis (NASH) carries an increased risk of cardiovascular disease (CVD) relative to the general population. We sought to evaluate whether differences in lipoprotein subfractions in obese patients with and without NASH contributes to this difference in CVD risk.
Ion mobility analysis was performed on 78 individuals with obesity undergoing weight loss surgery. All individuals had standard of care liver biopsies performed during surgery. Patients with NASH had significantly smaller peak LDL diameter (P = 0.02, 219.0 Å vs. 222.6 Å), and levels of IDL2 (P = 0.01, 104. nmol/L vs. 133.4 nmol/L) and HDL2b (P = 0.05, 676.7 nmol/L vs. 880.1 nmol/L) compared to those without NASH. NASH patients had significantly higher LDL-IVb levels than those without NASH (P = 0.02, 49.0 nmol/L vs. 37.1 nmol/L).
The inverse association of LDL peak diameter with NASH remained significant after adjustment for diabetes (P = 0.02). HDL2b levels were inversely correlated with hepatocyte ballooning and NASH and these remained significant after adjustment for diabetes (P = 0.0017 and P = 0.007, respectively). IDL2 levels were inversely correlated with NASH, hepatocyte ballooning and fibrosis stage but these were not significant after adjustment for diabetes.
The lipoprotein subfraction profile in subjects with NASH is characterized by small peak LDL diameter, reduced HDL2b levels and elevated LDL-IVb levels. These changes may contribute to the increased CVD seen in patients with NASH.