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Open Access Research

Protective effects of Sonchus asper against KBrO3 induced lipid peroxidation in rats

Rahmat Ali Khan1*, Muhammad Rashid Khan2 and Sumaira Sahreen3

Author Affiliations

1 Department of Biotechnology, Faculty of Sciences, University of Science and Technology Bannu, Bannu, Pakistan

2 Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan

3 Botanical Sciences Division, Pakistan Museum of Natural History, Islamabad, Pakistan

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Lipids in Health and Disease 2012, 11:164  doi:10.1186/1476-511X-11-164

Published: 27 November 2012

Abstract

Background

Sonchus asper is traditionally used in Pakistan for the treatment of reproductive dysfunction and oxidative stress. The present investigation was aimed to evaluate chloroform extract of Sonchus asper (SACE) against potassium bromate-induced reproductive stress in male rats.

Methods

20 mg/kg body weight (b.w.) potassium bromate (KBrO3) was induced in 36 rats for four weeks and checked the protective efficacy of SACE at various hormonal imbalances, alteration of antioxidant enzymes, and DNA fragmentation levels. High performance chromatography (HPLC) was used for determination of bioactive constituents responsible.

Results

The level of hormonal secretion was significantly altered by potassium bromate. DNA fragmentation%, activity of antioxidant enzymes; catalase (CAT), peroxidase (POD), superoxide dismutase (SOD) and phase II metabolizing enzymes viz; glutathione reductase (GSR), glutathione peroxidase (GSHpx), glutathione-S-tansase (GST) and reduced glutathione (GSH) was decreased while hydrogen per oxide contents and thiobarbituric acid reactive substances (TBARS) were increased with KBrO3 treatment. Treatment with SACE effectively ameliorated the alterations in the biochemical markers; hormonal and molecular levels while HPLC characterization revealed the presence of catechin, kaempferol, rutin and quercetin.

Conclusion

Protective effects of Sonchus asper vs. KBrO3 induced lipid peroxidation might be due to bioactive compound present in SACE.

Keywords:
Potassium bromate; Sonchus asper; Antioxidant; FSH; DNA fragmentation; TSH