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Dietary plasmalogen increases erythrocyte membrane plasmalogen in rats

Shiro Mawatari1*, Toshihiko Katafuchi2, Kiyotaka Miake3 and Takehiko Fujino4

Author Affiliations

1 Institute of Rheological Function of Food, 2241 Kubara, Hisayama-chou, Kasuya-gun, Fukuoka, 811-2501, Japan

2 Department of Integrative Physiology, Graduate School of Medical Science Kyushu University, Fukuoka, 812-8582, Japan

3 Central Research Institute, Marudai Food Co. Ltd, Osaka, Japan

4 Institute of Rheological Function of Food, 2241 Kubara, Hisayama-chou, Kasuya-gun, Fukuoka, 811-2501, Japan

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Lipids in Health and Disease 2012, 11:161  doi:10.1186/1476-511X-11-161

Published: 21 November 2012

Abstract

Background

Many disorders with plasmalogen deficiency have been reported. Replenishment or replacement of tissue plasmalogens of these disorders would be beneficial to the patients with these disorders, but effects of dietary plasmalogen on mammals have not been reported.

Methods

Plasmalogens were purified from chicken skin. The purified plasmalogens consisted of 96.4% ethanolamine plasmalogen (PlsEtn), 2.4% choline plasmalogen (PlsCho) and 0.5% sphingomyelin (SM). A diet containing 0.1% the purified plasmalogens (PlsEtn diet) was given to rats. Relative composition of phospholipids was measured by a high performance liquid chromatography (HPLC) method that can separate intact plasmalogens and all other phospholipid classes by a single chromatographic run.

Results

The PlsEtn diet given to Zucker diabetic fatty (ZDF) rats for 4 weeks caused decreases of plasma cholesterol and plasma phospholipid as compared to control diet. The other routine laboratory tests of plasma including triacylglycerol, glucose, liver and renal functions, albumin, and body weight were not different. Relative compositions of erythrocyte PlsEtn and phosphatidylethanolamine (PE) increased, and that of phosphatidylcholine (PC) decreased in PlsEtn diet group. The PlsEtn diet given to normal rats for 9 weeks again caused decrease of plasma cholesterol and phospholipid, and it induced increase of relative composition of PlsEtn of the erythrocyte membrane. The other routine laboratory tests of plasma and body weight were not different.

Conclusions

Dietary PlsEtn increases relative composition of PlsEtn of erythrocyte membranes in normal and ZDF rats, and it causes decreases of plasma cholesterol and plasma phospholipids. Dietary PlsEtn for 9 weeks seemingly causes no adverse effect to health of normal rats.

Keywords:
Dietary plasmalogen; Zucker diabetic fatty rat; Wistar rat; Erythrocyte phospholipids; Plasma phospholipids