eNOS genotype modifies the effect of leisure-time physical activity on serum triglyceride levels in a Japanese population
1 Department of Preventive Medicine, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan
2 Department of Public Health, Nagoya City University Graduate School of Medical Sciences, Nagoya, 467-8601, Japan
3 Department of Health Science, Shiga University of Medical Science, Otsu, 520-2192, Japan
4 Department of International Island and Community Medicine, Kagoshima University Graduate School of Medical and Dental Science, Kagoshima, 890-8544, Japan
5 Department of Preventive Medicine, Faculty of Medicine, Saga University, Saga, 849-8501, Japan
6 Department of Geriatric Medicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka, 812-8582, Japan
7 Department of Preventive Medicine, Institute of Health Biosciences, the University of Tokushima Graduate School, Tokushima, 770-8503, Japan
8 Department of Epidemiology for Community Health and Medicine, Kyoto Prefectural University of Medicine, Kyoto, 602-8566, Japan
9 Division of Epidemiology and Prevention, Aichi Cancer Center Research Institute, Nagoya, 464–8681, Japan
10 Division of Cancer Registry, Prevention and Epidemiology, Chiba Cancer Center, Chiba, 260-8717, Japan
11 Laboratory for Genotyping Development, Center for Genomic Medicine, RIKEN, Yokohama, 230-0045, Japan
Lipids in Health and Disease 2012, 11:150 doi:10.1186/1476-511X-11-150Published: 5 November 2012
Nitric oxide is a key molecule not only in the cardiovascular system, but also in the metabolic-endocrine system. The purpose of this study was to examine possible associations of the NOS3 T-786C polymorphism (rs2070744) with serum lipid levels on the basis of lifestyle factors for tailoring prevention of dyslipidemia.
For this cross-sectional study, a total of 2226 subjects aged 35 to 69 years (1084 men and 1142 women) were selected from Japanese participants in the Japan Multi-Institutional Collaborative Cohort (J-MICC) Study. They were recruited in eight areas throughout Japan between February 2004 and November 2008.
In a stratified analysis by leisure-time physical activity, the likelihood of hypertriglyceridemia (serum triglyceride levels ≥ 150 mg/dL) among subjects with the C allele was significantly lower than those without it in the active group (OR = 0.43, 95% CI = 0.22-0.84 in the fasting group), but not in the sedentary group. A gene-environment interaction between the T-786C polymorphism and leisure-time physical activity for hypertriglyceridemia was significant (P = 0.007 in the fasting group). Additionally, serum triglyceride levels (mean ± SD) across leisure-time physical activity classes decreased significantly only in the TC + CC genotype group (111 ± 60 mg/dL for sedentary, 95 ± 48 mg/dL for moderately active, 88 ± 44 mg/dL for very active, P for trend = 0.008 in the fasting group), but not in the TT genotype group. Total cholesterol, high-density lipoprotein (HDL) cholesterol, and non-HDL cholesterol levels had no significant association with the polymorphism.
This study suggests that the NOS3 T-786C polymorphism modifies the effect of leisure-time physical activity on serum triglyceride levels.