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Effects of physical training with different intensities of effort on lipid metabolism in rats submitted to the neonatal application of alloxan

Carla Ribeiro*, Lucieli Teresa Cambri, Rodrigo Augusto Dalia, Michel Barbosa de Araújo, José Diego Botezelli, Amanda Christine da Silva Sponton and Maria Alice Rostom de Mello

Author Affiliations

Institute of Biosciences Physical Education Department, São Paulo State University – (Universidade Estadual Paulista- UNESP), Av: 24-A, 1515 Bela Vista, Rio Claro – São Paulo, CEP: 13506-900, Brazil

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Lipids in Health and Disease 2012, 11:138  doi:10.1186/1476-511X-11-138

Published: 15 October 2012



Type 2 diabetes mellitus (T2DM) is a chronic disease that is characterized by insulin resistance. Its development is directly connected with the inability of insulin to exert its action, not just on carbohydrate metabolism but also on primarily on lipid metabolism. The present study aimed to compare the effects of continuous, intermittent, and strength training on serum and tissue variables on the lipid metabolism of alloxan rats.


Wistar rats were divided into eight groups: sedentary alloxan (SA), sedentary control (SC), continuous training alloxan (CA), intermittent training alloxan (IA), strength training alloxan (StA), continuous training control (CC), intermittent training control (IC) and strength training control (StC). Alloxan (250 mg/kg bw) was injected into neonatal rats at 6 days of age. The continuous training protocol consisted of 12 weeks of swimming training for 1 uninterrupted hour / day, five days/ week, supporting a load that was 5% bw. The intermittent training protocol consisted of 12 weeks of swimming training with 30 s of activity interrupted by 30 s of rest, for a total of 20 min/day, five days/ week, supporting a load that was 15% bw. The strength-training protocol consisted of 12 weeks of training, five days/week with 4 sets of 10 jumps in water with 1 min rest between sets, supporting a load that was a 50% bw.


At 28 days, the alloxan animals exhibited higher insulin resistance as measured by the disappearance of glucose serum (% Kitt/min) during the ITT. At 120 days, the sedentary alloxan animals showed higher FFA values than continuous and intermittent training alloxan. In addition, the alloxan animals that underwent intermittent and strength training showed lower FFA values compared to the corresponding controls. The continuous training protocol was less effective than the strength training protocol for reducing the levels of total cholesterol in the alloxan animals. Serum total lipid values revealed that intermittent training increased serum levels in alloxan animals


Thus, it was concluded that physical training at different intensities of effort is of great importance in attenuation and control of changes in the lipid metabolism in alloxan animals.

Neonatal rats; Alloxan; Exercise training; Lipid metabolism