The I405V and Taq1B polymorphisms of the CETP gene differentially affect sub-clinical carotid atherosclerosis
1 Lipids Laboratory, Faculty of Medical Sciences, State University of Campinas, Rua 5 de Junho, 350, Campinas, SP 13083-877, Brazil
2 Lipids Laboratory, Biology Institute, State University of Campinas, Rua Monteiro Lobato, 255, Campinas, SP, 13083-862, Brazil
3 Faculty of Medicine, University of São Paulo, Av Bandeirantes, 3900, Ribeirão Preto, SP, 14039-900, Brazil
4 Cardiology Division, Faculty of Medical Sciences, State University of Campinas, Rua Tessália Vieria de Camargo, 126, Campinas, SP, 13083-887, Brazil
5 Imunology Department, University of São Paulo, Av Prof. Lineu Prestes, 2415, São Paulo, SP, 05508-900, Brazil
6 Department of Radiology, Faculty of Medical Sciences, State University of Campinas, Rua Tessália Vieira de Camargo, 126, Campinas, SP, 13083-887, Brazil
7 Lipids Laboratory (LIM 10), Faculty of Medical Sciences of the University of São Paulo, Av Dr Arnaldo, 455, São Paulo, SP, 01246-903, Brazil
8 Departamento de Patologia Clínica, FCM-UNICAMP, P.O. Box 6111, Rua Tessália Vieira de Camargo, 126, Campinas, Barão Geraldo, SP 13084-971, Brazil
Lipids in Health and Disease 2012, 11:130 doi:10.1186/1476-511X-11-130Published: 5 October 2012
Cholesteryl ester transfer protein (CETP) plays a major role in lipid metabolism, but studies on the association of CETP polymorphisms with risks of cardiovascular disease are inconsistent. This study investigated whether the CETP gene I405V and Taq1B polymorphisms modified subclinical atherosclerosis in an asymptomatic Brazilian population sample.
The polymorphisms were analyzed using polymerase chain reaction in 207 adult volunteers. Serum lipid profiles, oxLDL Ab titers, C-reactive protein and tumor necrosis factor-α concentrations and CETP and phospholipid transfer protein (PLTP) activities were determined, and common carotid artery intima-media thickness (cIMT) was measured using ultrasonography.
No differences in cIMT were observed between the presence or absence of the minor B2 and V alleles in either polymorphism. However, inverse correlations between mean cIMT and CETP activity in the presence of these polymorphisms were observed, and positive correlations of these polymorphisms with PLTP activity and oxLDL Ab titers were identified. Moreover, logistic multivariate analysis revealed that the presence of the B2 allele was associated with a 5.1-fold (CI 95%, OR: 1.26 – 21.06) increased risk for cIMT, which was equal and above the 66th percentile and positively interacted with age. However, no associations with the V allele or CETP and PLTP activities were observed.
None of the studied parameters, including CETP activity, explained the different relationships between these polymorphisms and cIMT, suggesting that other non-determined factors were affected by the genotypes and related to carotid atherosclerotic disease.