Research
MicroRNA profile of polyunsaturated fatty acid treated glioma cells reveal apoptosis-specific expression changes
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* Corresponding author: Nóra Faragó nora@avidinbiotech.com
1 Functional Genomics Laboratory, Biological Research Center of the Hungarian Academy of Sciences, Temesvári krt.62, Szeged H-6726, Hungary
2 Avidin Ltd., Közép fasor 52, Szeged H-6726, Hungary
3 UND Life Sciences, 13800 Fairhill Road, #321, Shaker Heights, OH 44120, USA
4 Jawaharlal Nehru Technological University, Kakinada-533 003, India
5 Krishna Institute of Medical Sciences, Secunderabad-500 003, India
6 Bio-Science Research Laboratory, Gayatri Vidya Parishad College of Engineering, Visakhapatnam-530 048, India
Lipids in Health and Disease 2011, 10:173 doi:10.1186/1476-511X-10-173
Published: 30 September 2011Abstract
Background
Polyunsaturated fatty acids (PUFAs) such as γ-linolenic acid (GLA), arachidonic acid (AA) and docosahexaenoic acid (DHA) have cytotoxic action on glioma cells.
Results
We evaluated the cytotoxic action of GLA, AA and DHA on glioma cells with specific reference to the expression of miRNAs. Relative expression of miRNAs were assessed by using high throughput nanocapillary real-time PCR. Most of the miRNA target genes that showed altered expression could be classified as apoptotic genes and were up-regulated by PUFA or temozolomide treatment, while similar treatments resulted in repression of the corresponding mRNAs, such as cox2, irs1, irs2, ccnd1, itgb3, bcl2, sirt1, tp53inp1 and k-ras.
Conclusions
Our results highlight involvement of miRNAs in the induction of apoptosis in glioma cells by fatty acids and temozolomide.