Anandamide uptake by synaptosomes from human, mouse and rat brain: inhibition by glutamine and glutamate

doi:10.1186/1476-511X-1-1

Lipids in Health and Disease

Volume 1

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Bari M
Finazzi-Agrò A
Maccarrone M

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Bari M
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Maccarrone M
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Short paper

Anandamide uptake by synaptosomes from human, mouse and rat brain: inhibition by glutamine and glutamate

Natalia Battista^* , Monica Bari^* , Alessandro Finazzi-Agrò and Mauro Maccarrone

Department of Experimental Medicine and Biochemical Sciences, University of Rome "Tor Vergata", Rome, Italy

author email corresponding author email* Contributed equally

Lipids in Health and Disease 2002, 1:1doi:10.1186/1476-511X-1-1


Published:	3 September 2002

Abstract

Anandamide (N-arachidonoylethanolamine, AEA) belongs to an emerging class of endogenous lipids, called "endocannabinoids". A specific AEA membrane transporter (AMT) allows the import of this lipid and its degradation by the intracellular enzyme AEA hydrolase. Here, we show that synaptosomes from human, mouse and rat brain might be an ideal ex vivo system for the study of: i) the accumulation of AEA in brain, and ii) the pharmacological properties of AMT inhibitors. Using this ex vivo system, we demonstrate for the first time that glutamine and glutamate act as non-competitive inhibitors of AEA uptake by human, mouse and rat brain AMT.